光纤生物传感器在HER3抗体药物定量检测中的应用

史健松; 于源华; 王美娇; 吴再辉; 石鑫; 张昊; 宫平; 嵇晓强

doi:10.3788/CO.20181103.0503

Volume 11 Issue 3

Jun. 2018

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Article Contents

Chinese Optics > 2018 > 11(3): 503-512.

SHI Jian-song, YU Yuan-hua, WANG Mei-jiao, WU Zai-hui, SHI Xin, ZHANG Hao, GONG Ping, JI Xiao-qiang. Application of optical fiber biosensor in quantitative detection of HER3 antibody[J]. Chinese Optics, 2018, 11(3): 503-512. doi: 10.3788/CO.20181103.0503

Citation:

SHI Jian-song, YU Yuan-hua, WANG Mei-jiao, WU Zai-hui, SHI Xin, ZHANG Hao, GONG Ping, JI Xiao-qiang. Application of optical fiber biosensor in quantitative detection of HER3 antibody[J]. Chinese Optics, 2018, 11(3): 503-512. doi: 10.3788/CO.20181103.0503

Citation:

SHI Jian-song, YU Yuan-hua, WANG Mei-jiao, WU Zai-hui, SHI Xin, ZHANG Hao, GONG Ping, JI Xiao-qiang. Application of optical fiber biosensor in quantitative detection of HER3 antibody[J]. Chinese Optics, 2018, 11(3): 503-512. doi: 10.3788/CO.20181103.0503

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Application of optical fiber biosensor in quantitative detection of HER3 antibody

doi: 10.3788/CO.20181103.0503

1.
School of Life and Science and Technology, Changchun University of Science and Technology, Changchun 130022, China
2.
Changchun University of Science and Technology College of Optical And Electronical Information, Changchun 130022, China

Funds:

Jilin Province Science and Technology Development Plan Project 20170309003YY

More Information

Corresponding author: JI Xiao-qiang, E-mail: zuoanmulan@163.com
Received Date: 30 Jan 2018
Rev Recd Date: 13 Mar 2018
Publish Date: 01 Jun 2018

Abstract

Abstract

In order to realize the real-time, sensitively and rapid monitoring of the interactions between biomolecules, and obtain kinetic parameters of biomolecules existence, concentration and interaction, an affinity detection method based on fiber biosensor is designed in this paper. Firstly, a coupling structure between a self-focusing lens and a quartz fiber is proposed for the coupling problem between the optical transmission system "Y"-type bifurcated optical fiber and the fiber probe in the optical interference bio-affinity sensing system. The coupling structure has an eccentricity tolerance of up to 0.02 mm, and a tilt tolerance of 0.1°. In order to solve the problem of high frequency noise of the interference spectrum, an improved EMD interference spectral signal processing method is adopted to effectively avoid the deviation of the extreme point position after filtering of interference spectral curves; at the same time, method for calculating the thickness of biomolecule film layer by using locally fitted extreme points is adopted to increase the resolution of the biofilm layer thickness to 50 pm. Using the established light interference bio-affinity detection system, a new method for the detection of concentrations of HER3-IgG1 antibody using gold nanoparticles for signal amplification was established. There was no need for cleaning and no cross-contamination will occur during the detection process. The experimental results show that the detection limit of the system reaches 0.0826μg/ml. Due to the advantages of short detection time, accurate measurement, high precision, and low cost, the system is suitable for the study of pharmacokinetics.
- fiber biosensor,
- HER3,
- bioaffinity,
- fiber coupling structure,
- EMD,
- pharmacokinetics

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References(16)

References

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