双色荧光辐射差分超分辨显微系统研究

张智敏; 匡翠方; 王子昂; 朱大钊; 陈友华; 李传康; 刘文杰; 刘旭

doi:10.3788/CO.20181103.0329

Volume 11 Issue 3

Jun. 2018

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Article Contents

Chinese Optics > 2018 > 11(3): 329-336.

ZHANG Zhi-min, KUANG Cui-fang, WANG Zi-ang, ZHU Da-zhao, CHEN You-hua, LI Chuan-kang, LIU Wen-jie, LIU Xu. Dual-color fluorescence emission difference super-resolution microscopy[J]. Chinese Optics, 2018, 11(3): 329-336. doi: 10.3788/CO.20181103.0329

Citation:

ZHANG Zhi-min, KUANG Cui-fang, WANG Zi-ang, ZHU Da-zhao, CHEN You-hua, LI Chuan-kang, LIU Wen-jie, LIU Xu. Dual-color fluorescence emission difference super-resolution microscopy[J]. Chinese Optics, 2018, 11(3): 329-336. doi: 10.3788/CO.20181103.0329

Citation:

ZHANG Zhi-min, KUANG Cui-fang, WANG Zi-ang, ZHU Da-zhao, CHEN You-hua, LI Chuan-kang, LIU Wen-jie, LIU Xu. Dual-color fluorescence emission difference super-resolution microscopy[J]. Chinese Optics, 2018, 11(3): 329-336. doi: 10.3788/CO.20181103.0329

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Dual-color fluorescence emission difference super-resolution microscopy

doi: 10.3788/CO.20181103.0329

College of Optical Science and Engineering, Zhejiang University, Hangzhou 310058, China

Funds:

National Key Scientific Instrument and Equipment Development Projects of China No.2016YFF0101401

More Information

Corresponding author: KUANG Cui-fang, E-mail:cfkuang@zju.edu.cn
Received Date: 11 Jan 2018
Rev Recd Date: 05 Mar 2018
Publish Date: 01 Jun 2018

Abstract

Abstract

To perform super-resolution imaging of different tissue structures of biological samples using fluorescence radiation differential microscopy simultaneously, a dual-color FED microscopy system is studied in this paper. The basic principle of the FED is to remove the confocal microscopy image obtained by scanning the solid spot from the confocal microscopy image obtained by scanning the hollow spot to obtain a super-resolution microscopy image. Based on the study of the monochromatic FED microscopy system, a feasible dual-color FED microscopy imaging system is proposed and imaging experiments are performed on fluorescent particles in this paper. The experimental results indicate that under excitation light of 488nm and 640nm, the system realizes spatial resolution of 135 nm and 160 nm of the fluorescent particles respectively. In addition, this system can also perform multi-color super-resolution microscopy imaging simultaneously on different tissues of biological samples, which meets the requirements of practical applications.
- optical microscopy,
- diffraction limit,
- fluorescence,
- fluorescence emission difference(FED),
- super-resolution

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References(20)

References

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