Volume 11 Issue 1
Feb.  2018
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XIU Jing-rui, HU Si-yi, LI Jin-hua, REN Sheng, LIU Li-wei. Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots[J]. Chinese Optics, 2018, 11(1): 74-82. doi: 10.3788/CO.20181101.0074
Citation: XIU Jing-rui, HU Si-yi, LI Jin-hua, REN Sheng, LIU Li-wei. Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots[J]. Chinese Optics, 2018, 11(1): 74-82. doi: 10.3788/CO.20181101.0074

Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots

doi: 10.3788/CO.20181101.0074
Funds:

Natural Science Foundation of SZU 2017027

National Natural Science Foundation of China 61722508

Changchun University of Science and Technology Innovation Fund XJJLG-2015-01

Changchun University of Science and Technology Youth Fund XQNJJ-2016-10

More Information
  • In this paper, a kind of fluorescence resonance energy transfer(FRET) system based on near infrared InP/ZnS Quantum Dots and fluorescence dye Cy7 was constructed, and the conversion efficiencies of FRET system at different pH values and different concentrations were measured. Experimental results indicated that when the concentration of quanium dots remained constant, the conversion efficiency of the system increased with the increasing of the concentration of dye. When the concentration ratio of InP/ZnS and Cy7 was 1:250, the conversion efficiency was 68%. The results of cell test showed that the FRET system had a high sensitivity to pH value, and the detection accuracy of pH value for cell microenvironment was 0.1, which could be used as a sensitive FRET probe for biological microenvironment detection.
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  • [1]
    胡珊. 荧光共振能量转移体系的研究及其在均相免疫分析中的应用[D]. 武汉: 华中科技大学, 2010. http://cdmd.cnki.com.cn/Article/CDMD-10487-1011036089.htm

    HU SH. Sudy on fluorescence resonance energy transfer system and its application in homogeneous immunoassay[D]. WuHan: Huazhong University of Science and Technology, 2010. (in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10487-1011036089.htm
    [2]
    FORSTER T. Intermolecular energy migration and fluorescence[J]. Ann. Physics, 1948, 2:55-75. http://www.oalib.com/references/7320187
    [3]
    郭尧君.荧光实验技术及其在分子生物学中的应用[M].北京:科学出版社, 1979.

    GUO Y J. Fluorescence Experimental Techniques and Their Applications in Molecular Biology[M]. Beijing:Science Press, 1979.(in Chinese)
    [4]
    LAKOWICZ J R. Energy Transfer:In Principles of Fluorescence Spectroscopy[M]. New York:Plenum Press, 1983.
    [5]
    CLEGG R. Fluorescence Imaging Spectroscopy and Microscopy[M]. NewYork:Wiley, 1996.
    [6]
    ANGELIS D A D. Fluorescence Resonance Energy Transfer Fret[M]. Encyclopedia of Medical Genomics and Proteomics.2004:1420.
    [7]
    FENG Y SH, LIU L W, HU S Y, et al.. Förster resonance energy transfer properties of a new type of near-infrared excitation PDT photosensitizer: CuInS2/ZnS quantum dots-5-aminolevulinic acid conjugates[J]. RSC Adv., 2016, 6:55568-55576. doi: 10.1039/C6RA06937A
    [8]
    FENG Y SH, LIU L W, HU S Y, et al.. Four-photon-excited fluorescence resonance energy transfer in an aqueous system from ZnSe:Mn/ZnS quantum dots to hypocrellin A[J]. Optics Express, 2016, 24(17):19627-19637. doi: 10.1364/OE.24.019627
    [9]
    KIKUCHI K, TAKAKUSA H, NAGANO T. Recent advances in the design of small molecule-based FRET sensors for cell biology[J]. Trends in Analytical Chemistry, 2004, 23(6):407-415. doi: 10.1016/S0165-9936(04)00608-9
    [10]
    JANSSEN A, BEERLING E, MEDEMA R, et al.. Intravital FRET imaging of tumor cell viability and mitosis during chemotherapy[J]. PLoS One, 2013, 8(5):e64029. doi: 10.1371/journal.pone.0064029
    [11]
    YUAN L, LIN W, ZHENG K, et al.. FRET-based small-molecule fluorescent probes:rational design and bioimaging applications[J]. Accounts of Chemical Research, 2013, 46(7):1462-1473. doi: 10.1021/ar300273v
    [12]
    TAO H L, LIAO X F, et al.. Determination of trace Hg2+ ions based on the fluorescence resonance energy transfer between fluorescent brightener and CdTe quantum dots[J]. Journal of Luminescence, 2014, 146: 376-381. doi: 10.1016/j.jlumin.2013.10.005
    [13]
    JIANG G F, TANG Y. A novel two-photon fluorescent probe for hydrogen sulfide in living cells using an acedan-NBD amine dyad based on FRET process with high selectivity and sensitivity[J]. New Journal of Chemistry, 2017, 41(14):6769-6774. doi: 10.1039/C7NJ01080J
    [14]
    WANG Y, SI B, LU S, et al.. Near-infrared excitation of CdTe quantum dots based on fluorescence resonance energy transfer and their use as fluorescent sensors[J]. Sensors & Actuators B Chemical, 2017, 246:127-135. https://www.sciencedirect.com/science/article/pii/S0925400516307079
    [15]
    张旭霞, 李斌, 张黎明, 等.有机-无机复合纳米材料的传感应用及机理[J].中国光学, 2015, 8(4):651-666. http://www.chineseoptics.net.cn/CN/abstract/abstract9330.shtml

    ZHANG X X, LI B, ZHANG L M, et al.. Sensing application and mechanism of organic-inorganic nanocomposites[J]. Chinese Optics, 2015, 8(4):651-666.(in Chinese) http://www.chineseoptics.net.cn/CN/abstract/abstract9330.shtml
    [16]
    翟英歌, 楚学影, 徐铭泽, 等.ZnS:Cu-罗丹明B的荧光共振能量转移性质[J].发光学报, 2017, 38(8):1028-1032. http://www.cqvip.com/QK/92489X/199302/1022267.html

    ZHAI Y G, CHU X Y, XU M Z, et al.. Properties of fluorescence resonance energy transfer of ZnS:Cu-rhodamine B[J]. Chinese Journal of Luminescence, 2017, 38(8):1028-1032.(in Chinese) http://www.cqvip.com/QK/92489X/199302/1022267.html
    [17]
    袁曦, 郑金桔, 李海波, 等.Mn掺杂ZnSe量子点变温发光性质研究[J].中国光学, 2015, 8(5):806-813. http://www.chineseoptics.net.cn/CN/abstract/abstract9349.shtml

    YUAN X, ZHENG J J, LI H B, et al.. Temperature-dependent photoluminescence properties of Mn-doped ZnSe quantum dots[J]. Chinese Optics, 2015, 8(5):806-813.(in Chinese) http://www.chineseoptics.net.cn/CN/abstract/abstract9349.shtml
    [18]
    王英帅, 周颖, 王珺楠, 等.金纳米棒核/二氧化硅壳纳米复合结构的可控制备及细胞成像[J].中国光学, 2013, 6(5):743-749. http://www.chineseoptics.net.cn/CN/abstract/abstract9059.shtml

    WANG Y SH, ZHOU Y, WANG J N, et al.. Controlled synthesis and cell imaging of gold nanorod-silica core-shell nanoparticles[J]. Chinese Optics, 20136(5):743-749.(in Chinese) http://www.chineseoptics.net.cn/CN/abstract/abstract9059.shtml
    [19]
    刘姝妤, 钟绵增, 孟秀清, 等.ZnO/ZnS核-壳量子点的双光子吸收效应[J].发光学报, 2015, 36(2):249-255. http://www.opticsjournal.net/abstract.htm?id=OJ150215000023PmSoVr

    LIU SH Y, ZHONG J Z, MENG X Q, et al.. Two-photon absorption in ZnO/ZnS core-shell quantum dots[J]. Chinese Journal of Luminescence, 2015, 36(2):249-255. http://www.opticsjournal.net/abstract.htm?id=OJ150215000023PmSoVr
    [20]
    刘惠玲. 纳米材料内部FRET体系的设计及在单/双光子光动力治疗中的潜在应用[D]. 南京: 南京工业大学, 2016. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y3108216

    LIU H L. Design of FRET system in nanomaterials and its potential application in single/two photon photodynamic therapy[D]. Nanjing: Nanjing University of Technology, 2016. (in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y3108216
    [21]
    REMEDIOS C G, MOENS P D. Fluorescence resonance energy transfer spectroscopy is a reliable "ruler" for measuring structural changes in proteins-dispelling the problem of the unknown orientation factor[J]. Journal of Structural Biology, 1995, 115(2):175-185. doi: 10.1006/jsbi.1995.1042
    [22]
    LIU L. Quantum dots:the new development of FRET[J]. Progress in Chemistry, 2006, 18(2):337-343. https://www.researchgate.net/publication/282707728_Quantum_dots_The_new_development_of_FRET
    [23]
    STEYER L, HAUGLANG R P. Energy transfer:a spectroscopic ruler[J]. Proceedings of the National Academy of Sciences of the United States of America, 1967, 58(2):719. doi: 10.1073/pnas.58.2.719
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