Volume 11 Issue 1
Feb.  2018
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XIU Jing-rui, HU Si-yi, LI Jin-hua, REN Sheng, LIU Li-wei. Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots[J]. Chinese Optics, 2018, 11(1): 74-82. doi: 10.3788/CO.20181101.0074
Citation: XIU Jing-rui, HU Si-yi, LI Jin-hua, REN Sheng, LIU Li-wei. Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots[J]. Chinese Optics, 2018, 11(1): 74-82. doi: 10.3788/CO.20181101.0074

Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots

doi: 10.3788/CO.20181101.0074

Natural Science Foundation of SZU 2017027

National Natural Science Foundation of China 61722508

Changchun University of Science and Technology Innovation Fund XJJLG-2015-01

Changchun University of Science and Technology Youth Fund XQNJJ-2016-10

More Information
  • In this paper, a kind of fluorescence resonance energy transfer(FRET) system based on near infrared InP/ZnS Quantum Dots and fluorescence dye Cy7 was constructed, and the conversion efficiencies of FRET system at different pH values and different concentrations were measured. Experimental results indicated that when the concentration of quanium dots remained constant, the conversion efficiency of the system increased with the increasing of the concentration of dye. When the concentration ratio of InP/ZnS and Cy7 was 1:250, the conversion efficiency was 68%. The results of cell test showed that the FRET system had a high sensitivity to pH value, and the detection accuracy of pH value for cell microenvironment was 0.1, which could be used as a sensitive FRET probe for biological microenvironment detection.
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