Volume 16 Issue 5
Sep.  2023
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Article Contents
FU Qiang, ZHANG Zhi-miao, ZHAO Shang-nan, LIU Yang, DONG Yang. Research progress of miniature head-mounted single photon fluorescence microscopic imaging technique[J]. Chinese Optics, 2023, 16(5): 1010-1021. doi: 10.37188/CO.2023-0007
Citation: FU Qiang, ZHANG Zhi-miao, ZHAO Shang-nan, LIU Yang, DONG Yang. Research progress of miniature head-mounted single photon fluorescence microscopic imaging technique[J]. Chinese Optics, 2023, 16(5): 1010-1021. doi: 10.37188/CO.2023-0007

Research progress of miniature head-mounted single photon fluorescence microscopic imaging technique

doi: 10.37188/CO.2023-0007
Funds:  Supported by National Natural Science Foundation of China (No. 62005271); Youth Innovation Promotion Association, CAS (No. 2021221); Youth growth technology program of Jilin province science and technology development plan (No. 20210508054RQ).
More Information
  • Corresponding author: fuqianghit@163.com
  • Received Date: 10 Jan 2023
  • Rev Recd Date: 05 Feb 2023
  • Accepted Date: 24 Mar 2023
  • Available Online: 05 May 2023
  • Miniature head-mounted single-photon fluorescence microscopy is a breakthrough approach for neuroscience research that has emerged in recent years. It can image the neural activity of freely moving vivo animals in real time, providing an unprecedented way to access neural signals and rapidly enhancing the understanding of how the brain works. Driven by the needs of brain science research, there have been many types of miniature head-mounted single-photon fluorescence microscopes, such as high-resolution imaging, wireless recording, 3D imaging, two-region imaging and two-color imaging. In order to have a more comprehensive understanding of this new optical neuroimaging technology, we classify its technologies according to the imaging field of view, introduce the characteristics of different types of micro-head-mounted single-photon fluorescence microscopes reported so far, and focus on the optical system scheme and optical performance parameters used. The advantages and disadvantages of different schemes are analyzed and compared and the future direction of development is described to provide reference for the practical application of brain science researchers.

     

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