Volume 14 Issue 6
Nov.  2021
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MENG Yong-kang, LEI Zhu-bing, MEI Qian, DONG Wen-fei. Development and application of urine biochemical detection system for a disc microfluidic chip[J]. Chinese Optics, 2021, 14(6): 1317-1326. doi: 10.37188/CO.2021-0067
Citation: MENG Yong-kang, LEI Zhu-bing, MEI Qian, DONG Wen-fei. Development and application of urine biochemical detection system for a disc microfluidic chip[J]. Chinese Optics, 2021, 14(6): 1317-1326. doi: 10.37188/CO.2021-0067

Development and application of urine biochemical detection system for a disc microfluidic chip

doi: 10.37188/CO.2021-0067
Funds:  Supported by National Key R&D Program of China (No. 2020YFC4500); National Natural Science Foundation of China (No. 21803075, No. 61805273, No. 81902166, No. 91959112, No. 62027825); Science and Technology Department of Jinan City (No. 2018GXRC016); Science Foundation of the Chinese Academy of Sciences (No. 2020SYHZ0041)
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  • Corresponding author: qmei@sibet.ac.cn
  • Received Date: 2021-03-26
  • Rev Recd Date: 2021-05-08
  • Available Online: 2021-06-02
  • Publish Date: 2021-11-19
  • In response to the need for the automated and rapid urine detection, microfluidic technology and biochemical analysis technology are adopted to design and fabricate a disc microfluidic chip used for urine biochemical detection. The chip consisted of microfluidic channels, capillary valve, siphon valve and ferrowax valve which can realize the sequential transportation of the sample and reagent, mixing and detection. COMSOL multiphysics software is used to model the structure of capillary valve and siphon valve and optimize the rotary frequency. Next a fully automatic urine biochemical detection system is generated based on a disc microfluidic chip. The effects of light source fluctuations and background interference on test results are reduced by dual optical path and dual wavelength detection. The detection system is characterized by urinary Retinol Binding Protein (RBP). The results demonstrate the Coefficient of Variation (CV) of the system is 1.3%−2.46%, indicating that the system has good repeatability. The calibration curve shows the linear correlation between urinary RBP concentration and the absorbance (R2=0.995). The four identical unit on the chip could perform a multi-sample or multi-parameter detection in parallel, in which it has a potential to be applied for the rapid detection of urinary protein.
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