Volume 14 Issue 6
Nov.  2021
Turn off MathJax
Article Contents
TANG Ling-yu, GE Ming-feng, DONG Wen-fei. Design and research of fully automatic push-broom hyperspectral microscopic imaging system[J]. Chinese Optics, 2021, 14(6): 1486-1494. doi: 10.37188/CO.2021-0040
Citation: TANG Ling-yu, GE Ming-feng, DONG Wen-fei. Design and research of fully automatic push-broom hyperspectral microscopic imaging system[J]. Chinese Optics, 2021, 14(6): 1486-1494. doi: 10.37188/CO.2021-0040

Design and research of fully automatic push-broom hyperspectral microscopic imaging system

doi: 10.37188/CO.2021-0040
Funds:  Supported by National Key R&D Program of China (No. 2017YFF0108600); Supported by the Scientific Instrument Developing Project of the Chinese Academy of Sciences (No.YJKYYQ20200038); Primary Research & Developement Plan of Jiangsu Province(Social Development No. BE2019683); The Science and Technology Department of Jinan City (No. 2018GXRC016)
More Information
  • Corresponding author: gemf@sibet.ac.cn
  • Received Date: 2021-02-19
  • Rev Recd Date: 2021-03-15
  • Available Online: 2021-06-02
  • Publish Date: 2021-11-19
  • To apply hyperspectral technology to the field of microscopic imaging more conveniently, we designed and built a fully automatic push-broom hyperspectral microscopic imaging system. In this system, an inverted microscope was designed as the main body, a prism-grating component was used for spectrum splitting, a high precision two-dimensional motorized stage was applied for a push-broom. A motor focus module was used to control the focus, and a hyperspectral microscopic image was collected through a highly sensitive sCMOS scientific camera. The system has the advantages of low cost, easy installation and adjustment, real-time focusing and large-field-of-view imaging. The spectral range of the system is from 420 nm to 800 nm to meet the spectrum detection requirements of most biological samples. The spectral resolution was better than 3.5 nm, and the spatial resolution was better than 0.87 μm through the monochromatic collimated light scanning calibration method. Then, the HE-stained breast cancer pathological slices was as the research object. The samples were investigated and compared using passive and active focusing for push-broom imaging. The advantages and disadvantages of the two focusing methods were analyzed and summarized. The results showed that both methods can meet the needs of large-field-of-view imaging, but active focus imaging is faster and clearer, and is more suitable for push-broom hyperspectral microscopy imaging systems. Through the design and research of a fully automatic push-broom hyperspectral microscopy imaging system, real-time focusing in hyperspectral microscopic imaging was realized and 3.25 mm×3.25 mm field of view imaging of biological samples with a 40X objective lens was achieved. This system could be beneficial for promoting the application of hyperspectral technology in the biomedical field.
  • loading
  • [1]
    SORG B S, MOELLER B J, DONOVAN O, et al. Hyperspectral imaging of hemoglobin saturation in tumor microvasculature and tumor hypoxia development[J]. Journal of Biomedical Optics, 2005, 10(4): 44004. doi: 10.1117/1.2003369
    LIU K X, LIN S F, ZHU S Q, et al. Hyperspectral microscopy combined with DAPI staining for the identification of hepatic carcinoma cells[J]. Biomedical Optics Express, 2021, 12(1): 173-180. doi: 10.1364/BOE.412158
    EADY M, PARK B. An unsupervised prediction model for salmonella detection with hyperspectral microscopy: a multi-year validation[J]. Applied Sciences,, 2021, 11(3): 895. doi: 10.3390/app11030895
    WANG J SH, LI Q L. Quantitative analysis of liver tumors at different stages using microscopic hyperspectral imaging technology[J]. Journal of Biomedical Optics,, 2018, 23(10): 106002.
    肖功海, 舒嵘, 薛永祺. 显微高光谱成像系统的设计[J]. 光学 精密工程,2004,12(4):367-372.

    XIAO G H, SHU R, XUE Y Q. Design of microscopic hyperspectral imaging system[J]. Optics and Precision Engineering, 2004, 12(4): 367-372. (in Chinese)
    李庆利, 薛永祺, 肖功海, 等. 显微高光谱成像的生物组织定量检测机理及方法研究[J]. 科学通报,2008,53(4):493-496. doi: 10.3321/j.issn:0023-074X.2008.04.018

    LI Q L, XUE Y Q, XIAO G H, et al. Research on the mechanism and method of biological tissue quantitative detection based on micro-hyperspectral imaging[J]. Chinese Science Bulletin, 2008, 53(4): 493-496. (in Chinese) doi: 10.3321/j.issn:0023-074X.2008.04.018
    ORTEGA S, GUERRA R, DÍAZ M, et al. Hyperspectral push-broom microscope development and characterization[J]. IEEE Access, 2019, 7: 122473-122491. doi: 10.1109/ACCESS.2019.2937729
    ORTEGA S, FABELO H, CAMACHO R, et al. Detecting brain tumor in pathological slides using hyperspectral imaging[J]. Biomedical Optics Express, 2018, 9(2): 818-831. doi: 10.1364/BOE.9.000818
    PU H B, LIN L, SUN D W. Principles of hyperspectral microscope imaging techniques and their applications in food quality and safety detection: a review[J]. Comprehensive Reviews in Food Science and Food Safety, 2019, 18(4): 853-866. doi: 10.1111/1541-4337.12432
    SELJEBOTN S T. Continuous autofocus for line scanning hyperspectral camera[D]. Trondheim: Norwegian University of Science and Technology, 2012.
    张佳伦, 郑玉权, 蔺超, 等. 消像散的自由曲面棱镜光谱仪光学系统设计[J]. 中国光学,2020,13(4):842-851. doi: 10.37188/CO.2019-0049

    ZHANG J L, ZHENG Y Q, LIN C, et al. Design of a freeform curved prism imaging spectrometer based on an anastigmatism[J]. Chinese Optics, 2020, 13(4): 842-851. (in Chinese) doi: 10.37188/CO.2019-0049
    张天一, 朱永田, 侯永辉, 等. LAMOST高分辨率光谱仪研制[J]. 中国光学,2019,12(1):148-155. doi: 10.3788/co.20191201.0148

    ZHANG T Y, ZHU Y T, HOU Y H, et al. Construction of a LAMOST high resolution spectrograph[J]. Chinese Optics, 2019, 12(1): 148-155. (in Chinese) doi: 10.3788/co.20191201.0148
    魏巍, 崔继承, 唐玉国, 等. 医用显微成像光谱仪的光谱定标技术[J]. 光学 精密工程,2016,24(5):1015-1020. doi: 10.3788/OPE.20162405.1015

    WEI W, CUI J CH, TANG Y G, et al. Spectral calibration of medical microscopic imaging spectrometer[J]. Optics and Precision Engineering, 2016, 24(5): 1015-1020. (in Chinese) doi: 10.3788/OPE.20162405.1015
    迟明波, 韩欣欣, 徐阳, 等. 宽谱段高分辨扫描光谱定标技术[J]. 中国光学,2020,13(2):249-257. doi: 10.3788/co.20201302.0249

    CHI M B, HAN X X, XU Y, et al. Broad band and high resolution scanning spectrum calibration technology[J]. Chinese Optics, 2020, 13(2): 249-257. (in Chinese) doi: 10.3788/co.20201302.0249
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(14)  / Tables(3)

    Article views (232) PDF downloads(41) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint