金纳米棒核/二氧化硅壳纳米复合结构的可控制备及细胞成像

王英帅; 周颖; 王珺楠; 邵丹; 孙允陆; 李晶; 董文飞

doi:10.3788/CO.20130605.0743

Volume 6 Issue 5

Oct. 2013

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Article Contents

Chinese Optics > 2013 > 6(5): 743-749.

WANG Ying-shuai, ZHOU Ying, WANG Jun-nan, SHAO Dan, SUN Yun-lu, LI Jing, DONG Wen-fei. Controlled synthesis and cell imaging of gold nanorod-silica core-shell nanoparticles[J]. Chinese Optics, 2013, 6(5): 743-749. doi: 10.3788/CO.20130605.0743

Citation:

WANG Ying-shuai, ZHOU Ying, WANG Jun-nan, SHAO Dan, SUN Yun-lu, LI Jing, DONG Wen-fei. Controlled synthesis and cell imaging of gold nanorod-silica core-shell nanoparticles[J]. Chinese Optics, 2013, 6(5): 743-749. doi: 10.3788/CO.20130605.0743

Citation:

WANG Ying-shuai, ZHOU Ying, WANG Jun-nan, SHAO Dan, SUN Yun-lu, LI Jing, DONG Wen-fei. Controlled synthesis and cell imaging of gold nanorod-silica core-shell nanoparticles[J]. Chinese Optics, 2013, 6(5): 743-749. doi: 10.3788/CO.20130605.0743

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Controlled synthesis and cell imaging of gold nanorod-silica core-shell nanoparticles

doi: 10.3788/CO.20130605.0743

1.
State Key Laboratory on Integrated Optoelectronics, Jilin University, Changchun 130012, China;
2.
Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, China;
3.
Department of Pharmacology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China

Received Date: 12 Jul 2013
Rev Recd Date: 17 Sep 2013
Publish Date: 10 Oct 2013

Abstract

Abstract

The template method and sol-gel technique were used for the preparation of gold nanorod-silica core-shell (GNR@SiO2) nanocomposites. The controllable synthesis, spectroscopic properties, cytotoxicity and cell imaging of these nanocomposites were discussed by using UV-Vis spectrophotometry, transmission electron microscopy, confocal microscopy and other characterized measurements. The results show that, by control of the reaction time, the thickness of silica shell can be facilely tuned in the regime of 20-30 nm; the colloidal stability of gold nanorods has been greatly improved, and their cell cytotoxicities are significantly decreased due to the presence of the silica shell. In addition, fluorescent probes can be easily attached on the silica shell by post chemical reactions. As formed fluorescent core-shell nanocomposites can be easily captured by cells through endocytosis pathway, which is useful for the optical imaging. Therefore, these nanoparticle composites have a great potential in fields of biosensors, cell imaging and photothermal therapy.
- gold nanorod,
- silica,
- core-shell structure,
- cytotoxicity,
- cell imaging

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[1] HUFF T B,TONG L,HANSEN M N,et al.. Hyperthermic effects of gold nanorods on tumor cells nanomedicine[J]. Nanomedicine,2007,2:125-132. [2] WANG L Y,BAI J W,HUANG Y,et al.. Multifunctional nanoparticles displaying magnetization and near-IR absorption Angew[J]. Chem.,2008,47:2439-2442. [3] HUANG X H,JAIN P K,L-SAYED M A,et al.. Gold nanoparticles and nanorods in medicine:from cancer diagnostics to photothermal therapy[J]. Nanomedicine,2007,2:681-693. [4] YU C X,IRUDAYARAJ J. Multiplex biosensor using gold nanorods[J]. Anal. Chem.,2007,79:572-579. [5] CHEN C D,CHENG S F,WANG C,et al.. Sensing capability of the localized surface plasmon resonance of gold nanorods[J]. Biosens. Bioelectron,2007,22:926-932. [6] DURR N J,LARSON T,SMITH D K,et al.. Two-photon luminescence imaging of cancer cells using molecularly targeted gold nanorods[J]. Nano Lett.,2007,7:941-945. [7] IMURA K,NAGAHARA T,OKAMOTO H. Dispersion of cylindrical surface plasmon polaritons[J]. J. Chem. Phys.,2005,122:154701-154705. [8] IMURA K,NAGAHARA T,OKAMOTO H. Characteristic near-field spectra of single gold nanorods and imaging of plasmon modes[J]. J. Phys. Chem. B,2005,109:13214-13220. [9] ZHANG Z J,WU X C,CHEN C Y,et al.. Mesoporous silica-coated gold Nanorods as a light-mediated multifunctional theranostic platform for cancer treatment[J]. Adv. Mat.,2012,24:1418-1423. [10] EL-SAYED M A. Some interesting properties of metals confined in time and nanometer space of different shapes[J]. Acc. Chem. Res.,2001,34:257-264. [11] GORELIKOV I,MATSUURA N. Single-step coating of mesoporous silica on cetyltrimethyl ammonium bromide-capped nanoparticles[J]. Nano Lett.,2008,8:369-373. [12] ZHANG L,ZHANG F,DONG W F,et al.. Magnetic-mesoprous Janus particles[J]. Chem. Commun.,2011,47:1225. [13] ZHANG L,ZHANG F,WANG Y SH,et al.. Magnetic colloidosomes fabricated by Fe₃O₄ SiO₂ hetero-nanorods[J]. Soft Matter,2011,7:7375-7381. [14] HUANG X H,EL-SAYED M A,QIAN W,et al.. Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods[J]. J. Am. Chem. Soc.,2006,128(6):2115-212. [15] 孙桂敏,杨培慧,孙俊环,等. 金纳米棒标记HepG2人肝癌细胞的荧光成像及其AFM探测[J]. 发光学报,2011,32(6):636-641. SUN G M,YANG P H,SUN J H,et al. Fluorescence imaging of hepatocellular carcinoma cells labeled with gold nanorods and detection by AFM[J]. Chinese J. Luminescence,2011,32(6):636-641.(in Chinese)

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