Volume 17 Issue 3
May  2024
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ZHANG Zhi-miao, WANG Cheng-miao, XIE Mian, LIN Yu, HAN Ye-ming, DENG Yong-bo, GUO Chang-liang, FU Qiang. Design of miniature head-mounted fluorescence microscope based on metalens[J]. Chinese Optics, 2024, 17(3): 512-520. doi: 10.37188/CO.2023-0237
Citation: ZHANG Zhi-miao, WANG Cheng-miao, XIE Mian, LIN Yu, HAN Ye-ming, DENG Yong-bo, GUO Chang-liang, FU Qiang. Design of miniature head-mounted fluorescence microscope based on metalens[J]. Chinese Optics, 2024, 17(3): 512-520. doi: 10.37188/CO.2023-0237

Design of miniature head-mounted fluorescence microscope based on metalens

doi: 10.37188/CO.2023-0237
Funds:  Supported by Youth Innovation Promotion Association, CAS (No. 2021221);Youth Growth Science and Technology Program of Jilin Province Science and Technology Development Plan (No. 20210508054RQ)
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  • Corresponding author: fuqianghit@163.com
  • Received Date: 27 Dec 2023
  • Rev Recd Date: 04 Jan 2024
  • Accepted Date: 06 Feb 2024
  • Available Online: 01 Mar 2024
  • The recent advent of miniature head-mounted fluorescence microscopes has revolutionized brain science research, enabling real-time imaging of neural activity in the brains of free-moving animals. However, the pursuit of miniaturization and reduced weight often results in a limited field of view, constraining the number of neurons observable. While larger field-of-view systems exist, their increased weight can impede the natural behaviors of the subjects. Addressing these limitations, a novel design utilizing a metalens schematic is proposed. This approach offers the benefits of being ultra-light, ultra-thin, and capable of high-quality imaging. By deriving the aberration formula specific to hyperbolic phase metalens and using it as a foundation, a design for a miniature fluorescence microscope was developed. This microscope boasts a 4 mm×4 mm field of view and a numerical aperture (NA) of 0.14, effectively correcting seven primary aberrations. The resulting prototype, weighing a mere 4.11 g, achieves a resolution of 7.8 μm across the entire field of view. This performance is sufficient to image neural activity in the brains of freely moving mice with single-cell resolution.

     

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