基于间接波前整形的近红外二区荧光共聚焦成像研究

谭天; 史天悦; 吴长锋; 彭洪尚

doi:10.37188/CO.2023-0070

Volume 17 Issue 1

Jan. 2024

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Chinese Optics > 2024 > 17(1): 150-159.

TAN Tian, SHI Tian-yue, WU Chang-feng, PENG Hong-shang. NIR-II fluorescence confocal imaging based on indirect wavefront shaping[J]. Chinese Optics, 2024, 17(1): 150-159. doi: 10.37188/CO.2023-0070

Citation:

TAN Tian, SHI Tian-yue, WU Chang-feng, PENG Hong-shang. NIR-II fluorescence confocal imaging based on indirect wavefront shaping[J]. Chinese Optics, 2024, 17(1): 150-159. doi: 10.37188/CO.2023-0070

TAN Tian, SHI Tian-yue, WU Chang-feng, PENG Hong-shang. NIR-II fluorescence confocal imaging based on indirect wavefront shaping[J]. Chinese Optics, 2024, 17(1): 150-159. doi: 10.37188/CO.2023-0070

Citation:

TAN Tian, SHI Tian-yue, WU Chang-feng, PENG Hong-shang. NIR-II fluorescence confocal imaging based on indirect wavefront shaping[J]. Chinese Optics, 2024, 17(1): 150-159. doi: 10.37188/CO.2023-0070

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NIR-II fluorescence confocal imaging based on indirect wavefront shaping

doi: 10.37188/CO.2023-0070

1.
Engineering Research Center of Photonic Design Software Ministry of Education, College of Science, Minzu University of China, Beijing 100081, China
2.
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China

Funds: Supported by the National Natural Science Foundation of China (No. 62175266, No. 62235007, No. 22204070); the Shenzhen Science and Technology Program (No. KQTD20170810111314625, No. JCYJ20210324115807021, No. SGDX20211123114002003); the Shenzhen Bay Laboratory (No. SZBL2021080601002); Guangdong Provincial Key Laboratory of Advanced Biomaterials (No. 2022B1212010003)

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Corresponding author: hshpeng@bjtu.edu.cn
Received Date: 18 Apr 2023
Rev Recd Date: 10 May 2023

Available Online: 22 Sep 2023

Abstract

Abstract

Optical aberrations caused by the scattering of biological tissues limit the imaging performance of optical systems. A near-infrared II fluorescence confocal imaging technique based on indirect wavefront shaping was investigated. First, we synthesized a highly efficient near-infrared II range fluorescent probe, where reducing the scattering of biological tissue can realize biopsy imaging with high-contrast. Second, we investigated the adaptive optical method based on indirect wavefront measurement. The indirect wavefront shaping technology was applied to the laser scanning confocal system, enabling the measurement and compensation of optical aberrations caused by biological tissues, and obtaining imaging of biological tissues with a high signal-to-noise ratio. Finally, near-infrared II fluorescence confocal imaging system based on indirect wavefront shaping was deployed and relevant experiments were conducted. The experimental results indicate that the system effectively compensates for the aberrations induced by air plates, scattering media and mouse skull, and increases the final signal intensity by 1.47, 1.95 and 2.85 times, respectively. As a result, the final imaging quality is significantly enhanced.
- Indirect wavefront shaping,
- near-infrared-II imaging,
- confocal imaging,
- in vivo experiments

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References(29)

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