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摘要: 多光子成像技术是一种层析能力好、信噪比高的新型光学成像技术。在皮肤光学三维检测中，多光子技术已经应用于无创在体成像，且已得到产业化开发。本文将首先介绍多光子皮肤检测系统的若干核心技术，即双光子自发荧光技术、二次谐波成像技术、荧光寿命成像技术、相干反斯托克斯-拉曼成像技术等，然后简要介绍多光子成像系统在皮肤疾病成像检测上的应用，最后分析该系统的优势和未来可能的发展趋势。
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关键词:
- 皮肤组织成像 /
- 多光子荧光成像 /
- 荧光寿命成像 /
- 相干反斯托克斯-拉曼 /
- 皮肤衰老检测
Abstract: Multi-photon imaging is a new technique for optical imaging with inherent optical sectioning abilities and a high signal-to-noise ratio. Multi-photon microscopy has applications in dermatological imaging for its non-invasive and in situ imaging capability. As such, it has been applied commercially. In this paper, we first introduce the core technologies of multiphoton microscopy including two-photon auto-fluorescence imaging, second-harmonic generation(SHG), fluorescence life time imaging(FLIM) and coherent anti-Stokes Raman scattering(CARS). We then introduce its application on skin imaging analysis. Finally, we analyze the advantages and the possible future development of this technology. -
图 2 健康人体皮肤活体成像，激发光波长760 nm：(a)角质层，0 μm深：角蛋白自发荧光信号；(b)颗粒层，20 μm深：角质细胞中的透明角质颗粒、NADPH、角蛋白自发荧光信号；(c)棘层，30 μm深：角质细胞密度增大；激发光波长800 nm; (d)真皮层，85 μm深：胶原蛋白、弹性蛋白自发荧光信号[11]
Figure 2. In vivo healthy human skin imaging with excitation wavelength of 760 nm:(a)Stratum corneum, 0 μm depth: auto-fluorescence signal of keratin; (b)Stratum granulosum, 20 μm depth: auto-fluorescence signal of keratohyalin granules, NADPH, and keratin in keratinocytes; (c)Stratum spinosum, 30 μm depth: increased cellular density of keratinocytes; 800 nm excitation wavelength: (d)Dermis, 85 μm depth: auto-fluorescence signal of collagen and elastin[11]
图 6 相干反斯托克斯-拉曼(CARS)的Jablonski图:(a)拉曼散射; (b)受激拉曼散射; (c)相干反斯托克斯-拉曼
Figure 6. Jablonski image of coherent anti-Stokes Raman scattering(CARS):(a)Raman Scattering; (b)Stimulated Raman Scattering; (c)Coherent Anti-Stokes Raman Scattering
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